Amelioration of Endotoxin-Induced Uveitis in Rabbit by Topical Administration of Tacrolimus Proglycosome Nano-Vesicles

This work was aimed to improve the efficacy of tacrolimus in treatment endotoxin-induced uveitis (EIU) using propylene glycol modified lipid vesicles termed as proglycosome nano-vesicles (PNVs). PNVs were prepared by film hydration method. Experimental rabbit eye induced an intravitreal injection 20 ?L endotoxin solution containing 100 ng lipopolysaccharide endotoxin. In vivo determined studying clinical symptoms slit lamp examination and quantitatively measuring levels tumor necrosis factor-alpha, interleukin-6, leukocytes total proteins aqueous humor, 24 h after Comparison made with healthy, untreated treated eyes. developed nano-sized, deformable showed sustained release over period 12 h. results indicated statistically significant difference between effects EIU compared tacrolimus. PNV not only subsides but also prevented breakdown blood barrier. Tacrolimus loaded are potential new topical for uveitis.